Transplantation of bone marrow mesenchymal stem cells affects the proliferation and function of CD4+T cells in mice

doi:10.3969/j.issn.2095-4344.2014.01.018

Abstract

Abstract:

BACKGROUND: Mesenchymal stem cells are found to have the immunoregulatory activities and a potential application prospect in the treatment of autoimmune diseases.
OBJECTIVE: To explore the mechanism of transplanting mesenchymal stems cells on the treatment of multiple sclerosis.
METHODS: The mouse mesenchymal stems cells were prepared, and injected into the allogenic and syngenic normal mice, to detect the frequency of CD4⁺CD25⁺Foxp3⁺T cells in the spleen, thymus, and lymph nodes by flow cytometry, and to detect the Foxp3, transforming growth factor-β1, and interleukin-10 mRNA in the spleen, thymus, and lymph nodes by reverse transcription-PCR.
RESULTS AND CONCLUSION: Transplantation of mesenchymal stem cells on normal mice led to a significant up-regulation of CD4⁺CD25⁺Foxp3⁺T cells, Foxp3, transforming growth factor-β1, and interleukin-10 mRNA in the spleen, thymus, and lymph nodes both in the allogenic and syngenic transplant groups. Transplantation of mesenchymal stem cells may be an available method in the treatment of autoimmune diseases, and CD4⁺CD25⁺Foxp3⁺T cell, Foxp3, transforming growth factor-β1, and interleukin-10 may be involved in this process.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, bone marrow, mesenchymal stem cells, cell transplantation, transplantation, isogeneic, autoimmunity

CLC Number:

R394.2

Su Shao-hong, Zhang Jun-feng, Li Qian-ru, Guan Sha-sha, Du Ying . Transplantation of bone marrow mesenchymal stem cells affects the proliferation and function of CD4+T cells in mice[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(1): 106-111.

Figures/Tables 2

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